Small and Large Molecule LC-MS

Liquid chromatography-mass spectrometry (LC-MS) is used for sensitive and selective quantitative bioanalysis across a range of drug modalities and analytes. Applications include pharmacokinetic (PK) studies, regulated bioanalysis, biomarker analysis and other bioanalytical applications throughout drug development.

LC-MS/MS using triple quadrupole mass spectrometry is widely used for targeted quantitative bioanalysis. HRMS adds high-resolution, accurate-mass capabilities that can provide greater analytical selectivity and help differentiate target analytes from potential interferences. The appropriate approach depends on the analyte, matrix, sensitivity requirements and objectives of the bioanalytical program.

Our scientists apply LC-MS bioanalytical expertise across a range of analytes and drug modalities, including:

• Small molecule drugs

• Peptides

• Proteins

• Oligonucleotides

• Lipids

• Biomarkers

• Antibody-conjugated modalities

These capabilities support the generation of quantitative bioanalytical data for regulatory submissions throughout drug development.

HRMS combines high mass resolution with accurate-mass measurement, providing increased analytical selectivity for challenging large molecule bioanalytical applications.

When working with complex molecules and biological matrices HRMS can provide additional analytical options by helping scientists:

• Differentiate target analytes from potential interferences using high-resolution, accurate-mass measurements

• Address complex bioanalytical challenges where increased analytical selectivity is needed

• Support quantitative bioanalysis of biological analytes using advanced LC-MS workflows

• Combine HRMS with low-flow chromatography for highly sensitive analytical approaches

• Apply multiplexed analytical strategies when multiple analytes need to be monitored within a single method

IQVIA Laboratories supports bioanalytical programs from early preclinical development through clinical development, Phase III and bioequivalence studies, with capabilities including assay development, method transfer, method validation and in-study sample analysis.