High complexity NGS workflows impact the ability to achieve consistent inter-laboratory performance. Often it is highly dependent on operator proficiency and institutional systems designed to minimize variability of testing performance. Here, we describe analytical performance and reproducibility of an automated next-generation sequencing (NGS) pan-cancer DNA & RNA assay at two global laboratory sites located in Durham, NC, USA and Beijing, China. The Oncomine™ Precision Assay on the Genexus™ Integrated Sequencer (OPAGX) is a targeted, NGS assay that detects biomarkers across 50 genes from both solid tissue and liquid biopsy samples. This assay utilizes unique molecular identifiers (UMIs) for error correction in sequencing and improved sensitivity for detection of single nucleotide variants (SNVs), small insertions or deletions, gene fusions, and amplifications in liquid biopsy samples.
Complete the form below to explore the methods, results, and conclusions of this study.
Related Insights
Reproducibility Challenges in an era of Massive Data
Presented at the MCBIOS & MAQC 2021 Joint Conference
A Verified Genomic Reference Sample for Assessing Performance of Cancer Panels Detecting Small Variants of Low Allele Frequency
Presented at the MCBIOS & MAQC 2021 Joint Conference
Unexpected Impacts to Library Complexity After DNA and RNA Library Preparation in Illumina Next Generation Sequencing
Presented at the MCBIOS & MAQC 2021 Joint Conference
Immune Landscape Signatures for Characterization of Tumor Microenvironment and Response to Therapy
Gene expression and analysis for detection of 38 Immune Landscape Signatures
Using Gene Signature Methodologies to Strengthen Immuno-Oncology Trials
Genomic technologies that empower and enhance immuno-oncology trials
Laboratory Solutions for Hepatitis B (HBV) Clinical Trials
Insight brief by Joseph Sebastian, Ph.D., Scientific Advisor, Infectious & Liver Diseases
Enabling blood sample prep for single cell sequencing in clinical research
The Role of Genomics in Clinical Trials with Patrick Hurban
I-O clinical development podcast series
IQVIA Laboratories Corporate Brochure
A global drug discovery and development laboratory services organization
Evaluating and Implementing SARS-CoV-2 PCR Tests for Diagnostic and Clinical Trial Use
AAPS 2020 COVID-19 Workshop: Current Pharmaceutical Developments for Cures and Prevention
