Insight
A Showcase of Ultra-Sensitive Peptide Hormone Quantitation
Measuring Human ACTH [1-39] at Low pg/mL Levels Using Three-Column Chromatography and HRMS
Ultra sensitive quantitation of peptide hormones in complex biological matrices remains a major challenge in LC MS bioanalysis. In this case study, IQVIA Laboratories scientists demonstrate a robust nano LC/HRMS workflow that achieves pg/mL level sensitivity for human ACTH (1–39) while maintaining system stability across extended sample runs. By incorporating a three column chromatography strategy, this approach overcomes common backpressure and robustness limitations of nano LC systems and enables reliable, high confidence peptide quantitation. Download the case study to explore the full workflow, performance data, and applicability to other low abundance endogenous peptides.
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